![]() ![]() The workbook contains questions for students to complete to enable staff to gauge student understanding. The tutorial can be completed within 2 h and I teach it in a workshop setting with a tutor on hand to provide practical assistance. It is suitable for computer-literate students (undergraduate and above) and for research staff. This record contains a self-paced tutorial to enable students to master the basic features of SciDAVis, of non-linear regression in general and to enable students to create a publication-quality figure. Commonly used pay-for software packages which carry out a similar function (and which are more powerful) include GraphPad Prism, SigmaPlot and Origin. ![]() From the Welcome dialog, choose the XY tab, drop the list of sample data sets and choose. SciDAVis is free to use and will carry out the basic analyses we need here adequately. Prism can easily fit a dose response curve to determine the IC50. In pharmacology, it is an important measure of potency for a given agent. Using the linear (ymx+n) or parabolic (yax 2 +bx+c) equation on this. This is by far the most common definition, and is usually what people mean by 'the IC50' of a response. To calculate the IC50 value: The inhibitor concentration against the percent activity is plotted ( I-Activity graph). This value is typically used to characterize an antagonist of a biological process (ex. When considering inhibitory data (measured response decreases with increasing concentration of antagonist), these two values are referred to as the Relative IC50 and Absolute IC50, respectively. Fitting data in this way enables us to extract parameters such as IC50 values and thus to compare the efficacy of different compounds in an experimental assay in a quantitative manner. IC50 Calculator IC 50 represents the concentration at which a substance exerts half of its maximal inhibitory effect. SciDAVis is a data fitting software package used to fit physically meaningful equations to experimental (assay) data using non-linear regression.
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